Biomarker-Identifizierung DMH 

1. Quantitative DNA Methylation Profiling on a High Density Oligonucleotide Microarray.
   Cancer gene profiling. Humana Press. Methods in Molecular Medicine. 2009, Christian Pilarsky and Robert Grützmann (Editor) ISBN: 978-1-934115-76-3.
   Fassbender A*, Lewin J, König T, Rujan T, Pelet C, Lesche R, Distler J and Schuster M.
2. Comparative DNA methylation analysis in normal and tumour tissues and in cancer cell lines using differential methylation hybridisation.
   Int J Biochem Cell Biol. 2007;39(7-8):1539-50.
   Lewin J, Plum A, Hildmann T, Rujan T, Eckhardt F, Liebenberg V, Lofton-Day C, Wasserkort R.
3. Methylation profiling of CpG islands in human breast cancer cells.
   Hum Mol Genet. 1999 Mar;8(3):459–70.
   Huang TH, Perry MR, Laux DE
4. Patent: WO2007/019670 Method and nucleic acids for the improved treatment of breast cancers.

Biomarker-Auswahl DBS 

1. DNA methylation profiling of human chromosomes 6, 20 and 22.
   Nat Genet. 2006 Dec;38(12):1378-85. Epub 2006 Oct 29.
   Eckhardt F, Lewin J, Cortese R, Rakyan VK, Attwood J, Burger M, Burton J, Cox TV, Davies R, Down TA, Haefliger C, Horton R, Howe K, Jackson DK, Kunde J, Koenig C, Liddle J, Niblett D, Otto T, Pettett R, Seemann S, Thompson C, West T, Rogers J, Olek A, Berlin K, Beck S.
2. Quantitative DNA methylation analysis based on four-dye trace data from direct sequencing of PCR amplificates.
   Bioinformatics. 2004 Nov 22;20(17):3005-12. Epub 2004 Jul 9.
   Lewin J, Schmitt AO, Adorján P, Hildmann T, Piepenbrock C.
3. DNA methylation profiling of transcription factor genes in normal lymphocyte development and lymphomas.
   Int J Biochem Cell Biol. 2007;39(7-8):1523-38.
   Ivascu C, Wasserkort R, Lesche R, Dong J, Stein H, Thiel A, Eckhardt F.
4. Generation of pluripotent stem cells from adult human testis.
   Nature. 2008 Nov 20;456(7220):344-9. Epub 2008 Oct 8.
   Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Bühring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T.
5. Patent: US 20040023279 , EP 1369493
6. HEP version of ESME software available under: http://www.epigenome.org/index.php?page=download

Biomarker-Validierung QM/HM 

1. A real-time PCR assay for DNA-methylation using methylation-specific blockers. Nucleic Acids Res. 2004 Jan 13;32(1):e10.
   Cottrell SE, Distler J, Goodman NS, Mooney SH, Kluth A, Olek A, Schwope I, Tetzner R, Ziebarth H, Berlin K.
2. Sensitive detection of colorectal cancer in peripheral blood by septin 9 DNA methylation assay.
   PLoS ONE. 2008;3(11):e3759. Epub 2008 Nov 19.
   Grützmann R, Molnar B, Pilarsky C, Habermann JK, Schlag PM, Saeger HD, Miehlke S, Stolz T, Model F, Roblick UJ, Bruch HP, Koch R, Liebenberg V, Devos T, Song X, Day RH, Sledziewski AZ, Lofton-Day C.
3. DNA methylation of the PITX2 gene promoter region is a strong independent prognostic marker of biochemical recurrence in patients with prostate cancer after radical prostatectomy.
   J Urol. 2009 Apr;181(4):1678-85. Epub 2009 Feb 23.
   Weiss G, Cottrell S, Distler J, Schatz P, Kristiansen G, Ittmann M, Haefliger C, Lesche R, Hartmann A, Corman J, Wheeler T.
4. DNA methylation biomarkers for blood-based colorectal cancer screening.
   Clin Chem. 2008 Feb;54(2):414-23. Epub 2007 Dec 18.
   Lofton-Day C, Model F, Devos T, Tetzner R, Distler J, Schuster M, Song X, Lesche R, Liebenberg V, Ebert M, Molnar B, Grützmann R, Pilarsky C, Sledziewski A.
5. Patent: WO 02/072880