Epi BiSKit – A complete kit for the preparation of purified, bisulfite-converted DNA
High-quality cell-free DNA is easily and robustly purified from up to 3,5 ml of plasma.
- Streamlined procedure for fast and reliable results
- Complete cytosine conversion
- DNA extraction system for highly sensitive analysis
The Epi BiSKit provides a complete set of reagents for the preparation of bisulfite-converted DNA from plasma derived from human whole blood samples. Using the reagents provided, each Epi BiSKit is intended to process 32 preparations, providing purified, sulfonated bisulfite-converted DNA for use in diverse, downstream molecular applications.
Summary and Principles
DNA is extracted from plasma by binding to magnetic beads. Impurities are removed from the magnetic beads by a wash step. The purified DNA is then released from the beads by suspension in an elution buffer, and treated with bisulfite reagents to produce sulfonated bisulfite converted DNA (bisDNA). The bisDNA is then re-purified using magnetic beads and cleansing steps to produce the finished product of pure, sulfonated bisulfite-converted DNA.
Biochemical process to yield sulfonated bisulfite-converted DNA
Bisulfite conversion is the method of choice for analyzing DNA methylation. It is based on the nucleophilic addition of a bisulfite ion to a cytosine nucleotide and a subsequent deamination reaction to yield uracil sulfonate; 5-methylcytosine (methylated cytosine) is protected from deamination by the methyl group. Thus, the sequence of bisDNA differs from the input DNA by the substitution of uracil nucleotides for unmethylated cytosines, while retaining methyl cytosine bases.
Kit Contents for Thirty-Two Plasma Preparations
|Lysis Binding Buffer||1 bottle||125.0 mL||15°C to 30°C|
|Wash A Concentrate||1 bottle||60.0 mL||15°C to 30°C|
|Wash B Concentrate||1 bottle||7.0 mL||15°C to 30°C|
|Magnetic Beads||1 bottle||4.0mL||15°C to 30°C|
|Bisulfite Solution||4 tubes||1.9 mL each||15°C to 30°C|
|Elution Buffer||1 tube||6.0 mL||15°C to 30°C|
|Protection Buffer||1 tube||1.0 mL||15°C to 30°C|
Please refer to the Instructions for Use for additional equipment and disposable supplies not included in the Epi BiSKit. There may be additional pre-analytical and analytical steps based upon the specific application that are not included in the Instructions for Use.
Instructions for Use:
Outside of US IFU 0019 PDF
US Only IFU 0020 PDF
In the United States, there is only “Research Use Only” Epi BiSKit available.
Safety data sheets PDF
Epi BiSKit (M7-01-001)